New method for determining total calcium content in tissue applied to skeletal muscle with and without calsequestrin
نویسندگان
چکیده
منابع مشابه
New method for determining total calcium content in tissue applied to skeletal muscle with and without calsequestrin
We describe a new method for determining the concentration of total Ca in whole skeletal muscle samples ([CaT]WM in units of mmoles/kg wet weight) using the Ca-dependent UV absorbance spectra of the Ca chelator BAPTA (1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid). Muscle tissue was homogenized in a solution containing 0.15 mM BAPTA and 0.5% sodium dodecyl sulfate (to permeabilize me...
متن کاملParadoxical buffering of calcium by calsequestrin demonstrated for the calcium store of skeletal muscle
Contractile activation in striated muscles requires a Ca(2+) reservoir of large capacity inside the sarcoplasmic reticulum (SR), presumably the protein calsequestrin. The buffering power of calsequestrin in vitro has a paradoxical dependence on [Ca(2+)] that should be valuable for function. Here, we demonstrate that this dependence is present in living cells. Ca(2+) signals elicited by membrane...
متن کاملCalsequestrin, triadin and more: the molecules that modulate calcium release in cardiac and skeletal muscle.
Calcium signals play a role in every cell where functional switching takes place. These signals were demonstrated first in striated muscles, where they control the force-generating interaction among contractile filaments, the function named excitation–contraction (EC) coupling. The three main molecular players that cooperate to produce these signals are the Ca2+ release channel of the sarcoplas...
متن کاملD4cpv-calsequestrin: a sensitive ratiometric biosensor accurately targeted to the calcium store of skeletal muscle
Current fluorescent monitors of free [Ca(2+)] in the sarcoplasmic reticulum (SR) of skeletal muscle cells are of limited quantitative value. They provide either a nonratio signal that is difficult to calibrate and is not specific or, in the case of Forster resonant energy transfer (FRET) biosensors, a signal of small dynamic range, which may be degraded further by imperfect targeting and interf...
متن کاملCalsequestrin targeting to sarcoplasmic reticulum of skeletal muscle fibers.
Calsequestrin (CS) is the low-affinity, high-capacity calcium binding protein segregated to the lumen of terminal cisternae (TC) of the sarcoplasmic reticulum (SR). The physiological role of CS in controlling calcium release from the SR depends on both its intrinsic properties and its localization. The mechanisms of CS targeting were investigated in skeletal muscle fibers and C2C12 myotubes, a ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 2015
ISSN: 1540-7748,0022-1295
DOI: 10.1085/jgp.201411250